Biology to barcodes™
TempO-Seq™
BioSpyder Technologies Inc. has developed a novel product for targeted sequencing called TempO-Seq™, a gene expression profiling tool designed to monitor hundreds to thousands of genes at once in high throughput. TempO-Seq can analyze expression in samples with thousands of cells or from single cells without pre-amplification, maximizing utilization of precious or limited samples. Sample barcoding, together with sequencing short fragments of each gene, enables pooling up to 6,144 samples in one sequencing run. Assay content is flexible and customizable delivering unprecedented accuracy and sensitivity with simplified data analysis that eliminates the need for bioinformatics. TempO-Seq assays deliver an easy to use solution for customers doing expression profiling for any species.
Contact us
Contact us for more information on how to implement molecular endpoints to your high-throughput studies.
Panels
Whole transcriptome or surrogate for human, mouse, rat, and zebrafish
FFPE assay
Profile FFPE samples efficiently without nucleic acid extraction or purification.
TempO-SeqR
‘Omic data analysis made easy with our biologist-friendly GUI-based analysis tool
Covering the biological universe
Simultaneously analyze thousands of targets
TempO-Seq turns Biology to Barcodes™, bringing the power of targeted sequencing to high-throughput sample processing with the use of template-dependent oligo ligation. The process is both simple and ingenious. Detector oligos bind to specific RNA sequences and unbound oligos are eliminated. After the detectors are ligated and amplified with sample-tagged primers, the library is pooled and sequenced.
Applications
Bring whole transcriptome profiling to your lab.
TempO-Seq opens new worlds of science to almost any genomics lab. Suddenly large scale studies are both affordable and practical, thanks to high target content, high sample throughput, and simple data analysis.
Specifications
MORE AFFORDABLE
pooling samples and multiplexing thousands of genes makes sequencing extremely inexpensive
Sequence up to 6,144 samples in one run
FASTER
one day assay with <1 h hands-on time
simple, streamlined bioinformatics analysis
FLEXIBLE INPUTS
cell lysates, FFPE or frozen tissue, low input (down to 10pg)
DNA,RNA, protein and more
DEEPER INSIGHTS
analyze thousands of targets while processing hundreds to millions of samples add or subtract targets without any impact on data
SIMPLE
no DNA conversion: cell lysates, purified RNA, or frozen/FFPE tissue can go directly in the assay
robust protocol for all sample types
MODULAR AND SCALEABLE
whole transcriptome panels, S1500+ surrogate panels, custom panels: add or subtract gene targets
with no impact to other targets in your signature
FREQUENTLY ASKED QUESTIONS
-
Please email us at info@biospyder.com.
-
We typically complete a service project in 4-6 weeks after receipt of samples.
-
TempO-Seq can be run on cell lysates, isolated RNA, whole blood, blood spotted on filter paper, and FFPE tissue slides, and others. Please contact us to learn more about what samples we can accept.
For more frequently asked questions, visit our FAQ page.