Tempo-seq™
Key features
input down to 10 pg with single-base specificity
no RNA extraction, cDNA conversion or preamplification
no proprietary instrumentation required: standard PCR cycler or microplate incubator is all you need
maximize sequence capacity due to short barcode reads: up to 6,144 samples in one run
“off the shelf” whole transcriptome or surrogate panels - or fully customizeable panels assessing any number of isoforms, gene fusions, mutations, or SNPs in any species
Sensitive Gene Expression Profiling with TempO-Seq: Advantages and Applications (White Paper)
Assay workflow and chemistry
TempO-Seq (Templated Oligo-Sequencing) is based on hybridization and sequencing of highly specific detector oligos.
Limitations of RNA-Seq
RNA-Seq is a powerful tool for discovery of new gene variants but suffers from limitations in terms of throughput, cost, and practicality.
Detector oligo design
Our oligo design pipeline maximizes isoform capture and specificity.
Data analysis
Data analysis is greatly simplified with TempO-Seq data.
Publications & presentations
Watch online webinars and read the peer-reviewed scientific literature demonstrating TempO-Seq sensitivity and specificity.