High-throughput gene expression profiling made fast, affordable and practical
BioSpyder Technologies has developed a novel product for targeted sequencing called TempO-Seq™, a gene expression profiling tool designed to monitor hundreds to thousands of genes at once in high throughput. TempO-Seq can analyze expression in samples with thousands of cells or from single cells without pre-amplification, maximizing utilization of precious or limited samples. Sample barcoding, together with sequencing short fragments of each gene, enables pooling up to 6,144 samples in one sequencing run. Assay content is flexible and customizable delivering unprecedented accuracy and sensitivity.with simplified data analysis that eliminates the need for bioinformatics. TempO-Seq assays deliver an easy to use solution for customers doing expression profiling for any species.
MORE AFFORDABLEPooling samples and multiplexing 1,000s of genes makes sequencing extremely inexpensive ; up to 6,144 samples in one sequencing run!
FASTER1 day Assay
Data takes minutes to analyze
SAMPLE FRIENDLY-Cell Lysate
-DNA, RNA, Proteins and more
-Minimal sample input ~10 picograms
DEEPER INSIGHTS- Analyze thousands of targets while processing hundreds to millions of samples
- Add or subtract targets without any impact on data
SIMPLE- No cDNA conversion
- Cell lysates or purified RNA
- Simple robust protocol for all sample types
MODULAR & SCALABLE- Whole transcriptions for any species
- Fixed panels
- Custom panels
- Add or subtract gene targets with no impact to other targets in your signature
Simultaneously analyze thousands of targets
TempO-Seq brings the power of targeted sequencing to high-throughput sample processing with the use of template-dependent oligo ligation.The process is both simple and ingenious. Detector oligos bind to specific RNA sequences and unbound oligos are eliminated. After the detectors are ligated and amplified with sample-tagged primers, the library is pooled and sequenced.READ MORE
APPLICATIONSBring whole transcriptome profiling to your lab.
TempO-Seq opens new worlds of science to almost any genomics lab. Suddenly large scale studies are both affordable and practical, thanks to high target content, high sample throughput, and simple data analysis. READ MORE