Introducing Tempo-seq™

 
 

Key features

  • input down to 10 pg with single-base specificity

  • no RNA extraction, cDNA conversion or preamplification

  • no proprietary instrumentation required: standard PCR cycler or microplate incubator is all you need

  • maximize sequence capacity due to short barcode reads: up to 6,144 samples in one run

  • “off the shelf” whole transcriptome or surrogate panels - or fully customizeable panels assessing any number of isoforms, gene fusions, mutations, or SNPs in any species

  • Sensitive Gene Expression Profiling with TempO-Seq: Advantages and Applications (White Paper)

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Assay workflow and chemistry

TempO-Seq (Templated Oligo-Sequencing) is based on hybridization and sequencing of highly specific detector oligos.

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Limitations of RNA-Seq

RNA-Seq is a powerful tool for discovery of new gene variants but suffers from limitations in terms of throughput, cost, and practicality.

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Detector oligo design

Our oligo design pipeline maximizes isoform capture and specificity.

 
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Data analysis

Data analysis is greatly simplified with TempO-Seq data.

Publications & presentations

Watch online webinars and read the peer-reviewed scientific literature demonstrating TempO-Seq sensitivity and specificity.