TempO-Seq FFPE assay

We have adapted TempO-Seq™ technology to profile gene expression in formalin-fixed paraffin-embedded (FFPE) tissue. This novel approach works by directly lysing tissue scraped from FFPE slides without the need to extract RNA or convert it to cDNA. Just as for crude cell lysates, TempO-Seq FFPE Assay is capable of targeted whole transcriptome, S1500+ Surrogate, or custom gene panel expression profiling from human, mouse, or rat while maintaining the sensitivity of purified RNA and cell lysate input. The TempO-Seq FFPE assay can also be customized to recognize custom gene panels and gDNA gene from all other mammalian species as well as bacteria and viruses. The FFPE assay has a high degree of reproducibility and produces gene expression profiles that correlate strongly with samples prepared fresh (R2 = .97). The assay can handle input areas of a wide range, and maintains sensitivity for input as small as 2mm^2 scraped from a 5 µm thick section. Further, the assay remains robust for samples that have undergone extended fixation times and samples that have been archived for more than 30 years.A thorough explanation of the assay and its capabilities are detailed in Trejo et al. (2019) PLoS ONE 14(2): e0212031.

Our FFPE assay was recently compared to other methods of analyzing transcriptomic profiles from clinical FFPE tissues in an independent study by investigators at the University of Edinburgh: Turnbull et al. (2020) BMC Bioinformatics 21(1), 1-10. Of note, TempO-Seq was the only approach which did not require prior RNA extraction.

Preparation of FFPE samples upstream of the TempO-Seq assay is described in a short video below.

If you would like to know more about our TempO-Seq FFPE assay or would like a quotation, please do not hesitate to contact us.