BioSpyder Technologies, Inc. has adapted TempO-Seq technology to profile gene expression in formalin-fixed paraffin-embedded (FFPE) tissue. This novel approach works by directly lysing tissue scraped from FFPE slides without the need to extract RNA or convert it to cDNA. The TempO-Seq FFPE Assay is capable of targeted whole transcriptome, S1500+ Surrogate, or custom gene panel expression profiling from human, mouse, or rat while maintaining the sensitivity of purified RNA and cell lysate input. The TempO-Seq FFPE assay can also be customized to recognize custom gene panels and gDNA gene from all other mammalian species as well as bacteria and viruses. The FFPE assay has a high degree of reproducibility and produces gene expression profiles that correlate strongly with samples prepared fresh (R2 = .97). The assay can handle input areas of a wide range, and maintains sensitivity for input as small as 2mm2 scraped from a 5 µm thick section. Further, the assay remains robust for samples that have undergone extended fixation times and samples that have been archived for more than 30 years.
A thorough explanation of the assay and its capabilities are detailed in the following publication: Click here.
Sample preparation upstream of standard TempO-Seq is demonstrated in the following video